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phelper plasmid  (TaKaRa)


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    Structured Review

    TaKaRa phelper plasmid
    Phelper Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aavpro+packaging+plasmid/AAVpro+Packaging+Plasmid/us12600969-701-1-6
    Average 94 stars, based on 40 article reviews
    phelper plasmid - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: After operation, atipamezole hydrochloride (5 mg/ml; Antisedan, Nihon Zenyaku Kogyo), diluted tenfold with saline, was administered at the rate of 0.75 ml/kg or 5.0 ml/kg body weight intraperitoneally for rat or mice, respectively, and awakening was performed on a 37 °C heating plate. .. AAV vectors were prepared with the AAVpro Helper Free System (AAV1, #6673, Takara Bio, Shiga, Japan), AAVpro Packaging Plasmid (AAV2, #6234; AAV5, #6664; AA6, #6665, Takara Bio), and AAVpro 293T Cell Line (#632273, Takara Bio). ..

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
    Article Snippet: .. AAV vectors were prepared with the AAVpro Helper Free System (AAV1, #6673, Takara Bio, Shiga, Japan), AAVpro Packaging Plasmid (AAV2, #6234; AAV5, #6664; AA6, #6665, Takara Bio), and AAVpro 293T Cell Line (#632273, Takara Bio). ..

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: After operation, atipamezole hydrochloride (5 mg/ml; Antisedan, Nihon Zenyaku Kogyo), diluted 10-fold with saline, was administered at the rate of 0.75 ml/kg or 5.0 ml/kg body weight intraperitoneally for rat or mice, respectively, and awakening was performed on a 37°C heating plate. .. Production Of Aav Vectors AAV vectors were prepared with the AAVpro Helper Free System (AAV1, #6673, Takara Bio, Shiga, Japan), AAVpro Packaging Plasmid (AAV2, #6234; AAV5, #6664; AA6, #6665, Takara Bio), and AAVpro 293T Cell Line (#632273, Takara Bio). ..

    Plasmid Preparation:

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: After operation, atipamezole hydrochloride (5 mg/ml; Antisedan, Nihon Zenyaku Kogyo), diluted tenfold with saline, was administered at the rate of 0.75 ml/kg or 5.0 ml/kg body weight intraperitoneally for rat or mice, respectively, and awakening was performed on a 37 °C heating plate. .. AAV vectors were prepared with the AAVpro Helper Free System (AAV1, #6673, Takara Bio, Shiga, Japan), AAVpro Packaging Plasmid (AAV2, #6234; AAV5, #6664; AA6, #6665, Takara Bio), and AAVpro 293T Cell Line (#632273, Takara Bio). ..

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
    Article Snippet: .. AAV vectors were prepared with the AAVpro Helper Free System (AAV1, #6673, Takara Bio, Shiga, Japan), AAVpro Packaging Plasmid (AAV2, #6234; AAV5, #6664; AA6, #6665, Takara Bio), and AAVpro 293T Cell Line (#632273, Takara Bio). ..

    Article Title: Engineering a highly durable adeno-associated virus receptor for analytical applications
    Article Snippet: .. AAV1, AAV2, AAV5, and AAV6 were prepared using the AAVpro Packaging Plasmid (Takara Bio, Shiga, Japan), while AAV4, AAV8, and AAV9 were synthesized by FASMAC Corporation (Kanagawa, Japan). .. In suspension cell culture, AAV was produced using a Gibco AAV-MAX production system (Thermo Fisher Scientific).

    Article Title: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
    Article Snippet: After operation, atipamezole hydrochloride (5 mg/ml; Antisedan, Nihon Zenyaku Kogyo), diluted 10-fold with saline, was administered at the rate of 0.75 ml/kg or 5.0 ml/kg body weight intraperitoneally for rat or mice, respectively, and awakening was performed on a 37°C heating plate. .. Production Of Aav Vectors AAV vectors were prepared with the AAVpro Helper Free System (AAV1, #6673, Takara Bio, Shiga, Japan), AAVpro Packaging Plasmid (AAV2, #6234; AAV5, #6664; AA6, #6665, Takara Bio), and AAVpro 293T Cell Line (#632273, Takara Bio). ..

    Synthesized:

    Article Title: Engineering a highly durable adeno-associated virus receptor for analytical applications
    Article Snippet: .. AAV1, AAV2, AAV5, and AAV6 were prepared using the AAVpro Packaging Plasmid (Takara Bio, Shiga, Japan), while AAV4, AAV8, and AAV9 were synthesized by FASMAC Corporation (Kanagawa, Japan). .. In suspension cell culture, AAV was produced using a Gibco AAV-MAX production system (Thermo Fisher Scientific).

    Article Title: Engineering a highly durable adeno-associated virus receptor for analytical applications
    Article Snippet: .. AAV1, AAV2, AAV5, and AAV6 were prepared using the AAVpro® Packaging Plasmid (Takara Bio, Shiga, Japan), while AAV4, AAV8, and AAV9 were synthesized by FASMAC (Kanagawa, Japan). .. AAV was produced using a Gibco AAV-MAX production system (Thermo Fisher Scientific).



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    TaKaRa aav packaging plasmid
    (A) Primary cortical neurons were used to test our <t>CreERT2</t> <t>(Addgene:</t> #210415) and DIO-mCherry constructs delivered using <t>AAV</t> transductions. (B) AAV DIO-mCherry produces minimal Cre-independent mCherry expression in PCNs. (C) Co-delivery of AAV-iCre (Addgene: #210416) and AAV DIO-mCherry to PCNs induces robust mCherry expression in PCNs. (D) AAV-CreERT2 and AAV DIO-mCherry co-transduction induces minimal tamoxifen-independent mCherry expression in PCNs, while addition of 4-hydroxytamoxifen (1µM) following the same AAV-transduction generates robust mCherry expression. (Scale Bar: 200 µm).
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    TaKaRa paav6 cmv plasmid
    (A) Primary cortical neurons were used to test our <t>CreERT2</t> <t>(Addgene:</t> #210415) and DIO-mCherry constructs delivered using <t>AAV</t> transductions. (B) AAV DIO-mCherry produces minimal Cre-independent mCherry expression in PCNs. (C) Co-delivery of AAV-iCre (Addgene: #210416) and AAV DIO-mCherry to PCNs induces robust mCherry expression in PCNs. (D) AAV-CreERT2 and AAV DIO-mCherry co-transduction induces minimal tamoxifen-independent mCherry expression in PCNs, while addition of 4-hydroxytamoxifen (1µM) following the same AAV-transduction generates robust mCherry expression. (Scale Bar: 200 µm).
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    Image Search Results


    (A) Primary cortical neurons were used to test our CreERT2 (Addgene: #210415) and DIO-mCherry constructs delivered using AAV transductions. (B) AAV DIO-mCherry produces minimal Cre-independent mCherry expression in PCNs. (C) Co-delivery of AAV-iCre (Addgene: #210416) and AAV DIO-mCherry to PCNs induces robust mCherry expression in PCNs. (D) AAV-CreERT2 and AAV DIO-mCherry co-transduction induces minimal tamoxifen-independent mCherry expression in PCNs, while addition of 4-hydroxytamoxifen (1µM) following the same AAV-transduction generates robust mCherry expression. (Scale Bar: 200 µm).

    Journal: bioRxiv

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    doi: 10.1101/2025.07.17.665447

    Figure Lengend Snippet: (A) Primary cortical neurons were used to test our CreERT2 (Addgene: #210415) and DIO-mCherry constructs delivered using AAV transductions. (B) AAV DIO-mCherry produces minimal Cre-independent mCherry expression in PCNs. (C) Co-delivery of AAV-iCre (Addgene: #210416) and AAV DIO-mCherry to PCNs induces robust mCherry expression in PCNs. (D) AAV-CreERT2 and AAV DIO-mCherry co-transduction induces minimal tamoxifen-independent mCherry expression in PCNs, while addition of 4-hydroxytamoxifen (1µM) following the same AAV-transduction generates robust mCherry expression. (Scale Bar: 200 µm).

    Article Snippet: The coding region for CreERT2 was amplified from MSCV CreERT2 puro (a gift from Tyler Jacks; Addgene; #22776) and inserted into an AAV packaging plasmid flanked by the EF1α promoter and the poly-adenylation signal from bovine growth hormone using ligation-independent cloning (In-Fusion, Clontech).

    Techniques: Construct, Expressing, Transduction

    (A) The DIO-mCherry rat uses human EF1α promoter to drive expression of mCherry fluorescent protein along with the woodchuck post transcriptional regulatory element (WPRE) and the human growth hormone polyadenylation signal (hGHpA). (A) The primer positions for a PCR genotyping assay spanning the 5’ junction of the mCherry cassette is noted by the black arrows and the positions of the primers (blue) and probe (green arrow) for a qPCR-based genotyping assay are also noted. ( B ) Copy number for WT, hemizygous (Hem), and homozygous (Hom) animals. The ratio is transgene copy number to the genomic gene (Ggt1) in genomic DNA samples isolated from the pups of two separate Hem × Hem crosses. The individual ratios of templates (DIO-mCherry/Ggt1) fall into three quanta (0, 0.5, and 1.0). (C) Amplicons (640 bp) produced by the 5’ junction PCR identify wild type (no bands) or carriers hemizygous and homozygous. (D) Images from the striatum of hemizygous DIO-mCherry rats injected with an AAV virus expressing Cre recombinase after two weeks. Low magnification (top row; scale bar: 500 μm) and higher magnification (bottom row; scale bar: 50 μm). Cre immunoreactivity (white) and mCherry epifluorescence (red) colocalize in both sets of images. Overlap Coefficient (OC)=0.90.

    Journal: bioRxiv

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    doi: 10.1101/2025.07.17.665447

    Figure Lengend Snippet: (A) The DIO-mCherry rat uses human EF1α promoter to drive expression of mCherry fluorescent protein along with the woodchuck post transcriptional regulatory element (WPRE) and the human growth hormone polyadenylation signal (hGHpA). (A) The primer positions for a PCR genotyping assay spanning the 5’ junction of the mCherry cassette is noted by the black arrows and the positions of the primers (blue) and probe (green arrow) for a qPCR-based genotyping assay are also noted. ( B ) Copy number for WT, hemizygous (Hem), and homozygous (Hom) animals. The ratio is transgene copy number to the genomic gene (Ggt1) in genomic DNA samples isolated from the pups of two separate Hem × Hem crosses. The individual ratios of templates (DIO-mCherry/Ggt1) fall into three quanta (0, 0.5, and 1.0). (C) Amplicons (640 bp) produced by the 5’ junction PCR identify wild type (no bands) or carriers hemizygous and homozygous. (D) Images from the striatum of hemizygous DIO-mCherry rats injected with an AAV virus expressing Cre recombinase after two weeks. Low magnification (top row; scale bar: 500 μm) and higher magnification (bottom row; scale bar: 50 μm). Cre immunoreactivity (white) and mCherry epifluorescence (red) colocalize in both sets of images. Overlap Coefficient (OC)=0.90.

    Article Snippet: The coding region for CreERT2 was amplified from MSCV CreERT2 puro (a gift from Tyler Jacks; Addgene; #22776) and inserted into an AAV packaging plasmid flanked by the EF1α promoter and the poly-adenylation signal from bovine growth hormone using ligation-independent cloning (In-Fusion, Clontech).

    Techniques: Expressing, Genotyping Assay, Isolation, Produced, Injection, Virus